Carbohydrates of lysosomal enzymes secreted by Tetrahymena pyriformis.
نویسندگان
چکیده
منابع مشابه
Purine-metabolizing enzymes of Tetrahymena pyriformis.
Except for the recent report of Friedkin on pyrimidine nucleoside phosphorylase (2) and several papers from this laboratory dealing with ribonuclease and deoxyribonuclease (3, 4), little information is available concerning the occurrence of nucleolytic and nucleosynthetic enzymes in the ciliated protozoan, Tetrahymena pyrif0rmis.l This is in contrast to a number of studies carried out on the pu...
متن کاملA NEW BIOLOGICAL SCREENING SYSTEM FOR LOCAL ANAESTHETICS BY INHIBITION MOBILITY OF TETRAHYMENA PYRIFORMIS
An alternative in vitro approach to drug screening has been the use of human cell cultures for antiviral agents and microbial cell cultures for the assessment of the carcinogenic potential of selected compounds. A number of protozoan species have been also used as drug screens for anti-protozoal agents. The ciliated protozoan Tetrahymena pyriformis species has been widely utilised as a dru...
متن کاملRibosome Synthesis in Tetrahymena Pyriformis
The cellular site of synthesis of ribosomal RNA in Tetrahymena pyriformis was studied by analyzing the purified nuclear and cytoplasmic RNA from cells pulse labeled with uridine-(3)H. The results of studies using zonal centrifugation in sucrose density gradients show that the ribosomal RNA is synthesized in the nucleus as a large precursor molecule sedimenting at 35S. The 35S molecule undergoes...
متن کاملStudies of Membrane Formation in Tetrahymena Pyriformis
A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
متن کاملThe metabolism of cyclopropane fatty acids by Tetrahymena pyriformis.
Cyclopropane fatty acids are readily metabolized by whole cells of Tetrahymena pyriformis when the fatty acids are presented either as components of intact Escherichia coli cells or as free fatty acids added to the medium. cis-ll,lZ[methylene-14C]Methyleneoctadecanoic acid was degraded with the production of 14COz by whole T. pyriformis cells or a particulate fraction derived from them. In the ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)38667-2